§ 136.3 Identification of test procedures.
(a) Parameters or pollutants, for which methods are approved, are listed together with test procedure descriptions and references in Tables IA, IB, IC, ID, IE, IF, IG, and IH of this section. The methods listed in Tables IA, IB, IC, ID, IE, IF, IG, and IH are incorporated by reference, see paragraph (b) of this section, with the exception of EPA Methods 200.7, 601-613, 624.1, 625.1, 1613, 1624, and 1625. The full texts of Methods 601-613, 624.1, 625.1, 1613, 1624, and 1625 are printed in appendix A of this part, and the full text of Method 200.7 is printed in appendix C of this part. The full text for determining the method detection limit when using the test procedures is given in appendix B of this part. In the event of a conflict between the reporting requirements of 40 CFR parts 122 and 125 and any reporting requirements associated with the methods listed in these tables, the provisions of 40 CFR parts 122 and 125 are controlling and will determine a permittee's reporting requirements. The full texts of the referenced test procedures are incorporated by reference into Tables IA, IB, IC, ID, IE, IF, IG, and IH. The date after the method number indicates the latest editorial change of the method. The discharge parameter values for which reports are required must be determined by one of the standard analytical test procedures incorporated by reference and described in Tables IA, IB, IC, ID, IE, IF, IG, and IH or by any alternate test procedure which has been approved by the Administrator under the provisions of paragraph (d) of this section and §§ 136.4 and 136.5. Under certain circumstances (paragraph (c) of this section, § 136.5(a) through (d) or 40 CFR 401.13,) other additional or alternate test procedures may be used.
Table IA—List of Approved Biological Methods for Wastewater and Sewage Sludge
| Parameter and units | Method 1 | EPA | Standard methods | AOAC, ASTM, USGS | Other |
|---|---|---|---|---|---|
| Bacteria | |||||
| 1. Coliform (fecal), number per gram dry weight | Most Probable Number (MPN), 5 tube, 3 dilution, or | p. 132 3, 1680 1115, 1681. 1120 | 9221 E-2014. | ||
| Membrane filter (MF) 25, single step | p. 124 3 | 9222 D-2015. 29 | |||
| 2. Coliform (fecal), number per 100 mL | MPN, 5 tube, 3 dilution, or | p. 132 3 | 9221 E-2014, 9221 F-2014. 33 | ||
| Multiple tube/multiple well, or | Colilert-18®. 131828 | ||||
| MF 25, single step 5 | p. 124 3 | 9222 D-2015. 29 | B-0050-85. 4 | ||
| 3. Coliform (total), number per 100 mL | MPN, 5 tube, 3 dilution, or | p. 114 3 | 9221 B-2014. | ||
| MF 25, single step or | p. 108 3 | 9222 B-2015. 30 | B-0025-85. 4 | ||
| MF 25, two step with enrichment | p. 111 3 | 9222 B-2015. 30 | |||
| 4. E. coli, number per 100 mL | MPN 6816 multiple tube, or | 9221 B2014/9221 F-2014. 121433 | |||
| multiple tube/multiple well, or | 9223 B-2016. 13 | 991.15 10 | Colilert®. 1318 Colilert-18®. 131718 | ||
| MF 25678, two step, or | 9222 B-2015/9222 I-2015. 31 | ||||
| Single step | 1603.1 21 | m-ColiBlue24®. 19 | |||
| 5. Fecal streptococci, number per 100 mL | MPN, 5 tube, 3 dilution, or | p. 139 3 | 9230 B-2013. | ||
| MF 2, or | p. 136 3 | 9230 C-2013 32 | B-0055-85. 4 | ||
| Plate count | p. 143 3 | ||||
| 6. Enterococci, number per 100 mL | MPN, 5 tube, 3 dilution, or | p. 139 3 | 9230 B-2013. | ||
| MPN 68, multiple tube/multiple well, or | 9230 D-2013 | D6503-99 9 | Enterolert®. 1323 | ||
| MF 25678 single step or | 1600.1 24 | 9230 C-2013. 32 | |||
| Plate count | p. 143. 3 | ||||
| 7. Salmonella, number per gram dry weight 11 | MPN multiple tube | 1682 22 | |||
| Aquatic Toxicity | |||||
| 8. Toxicity, acute, fresh water organisms, LC50, percent effluent | Water flea, Cladoceran, Ceriodaphnia dubia acute | 2002.0. 25 | |||
| Water flea, Cladocerans, Daphnia pulex and Daphnia magna acute | 2021.0. 25 | ||||
| Fish, Fathead minnow, Pimephales promelas, and Bannerfin shiner, Cyprinella leedsi, acute | 2000.0. 25 | ||||
| Fish, Rainbow trout, Oncorhynchus mykiss, and brook trout, Salvelinus fontinalis, acute | 2019.0. 25 | ||||
| 9. Toxicity, acute, estuarine and marine organisms of the Atlantic Ocean and Gulf of Mexico, LC50, percent effluent | Mysid, Mysidopsis bahia, acute | 2007.0. 25 | |||
| Fish, Sheepshead minnow, Cyprinodon variegatus, acute | 2004.0. 25 | ||||
| Fish, Silverside, Menidia beryllina, Menidia menidia, and Menidia peninsulae, acute | 2006.0. 25 | ||||
| 10. Toxicity, chronic, fresh water organisms, NOEC or IC25, percent effluent | Fish, Fathead minnow, Pimephales promelas, larval survival and growth | 1000.0. 26 | |||
| Fish, Fathead minnow, Pimephales promelas, embryo-larval survival and teratogenicity | 1001.0. 26 | ||||
| Water flea, Cladoceran, Ceriodaphnia dubia, survival and reproduction | 1002.0. 26 | ||||
| Green alga, Selenastrum capricornutum, growth | 1003.0. 26 | ||||
| 11. Toxicity, chronic, estuarine and marine organisms of the Atlantic Ocean and Gulf of Mexico, NOEC or IC25, percent effluent | Fish, Sheepshead minnow, Cyprinodon variegatus, larval survival and growth | 1004.0. 27 | |||
| Fish, Sheepshead minnow, Cyprinodon variegatus, embryo-larval survival and teratogenicity | 1005.0. 27 | ||||
| Fish, Inland silverside, Menidia beryllina, larval survival and growth | 1006.0. 27 | ||||
| Mysid, Mysidopsis bahia, survival, growth, and fecundity | 1007.0. 27 | ||||
| Sea urchin, Arbacia punctulata, fertilization | 1008.0. 27 | ||||
Table IA notes:
1 The method must be specified when results are reported.
2 A 0.45-µm membrane filter (MF) or other pore size certified by the manufacturer to fully retain organisms to be cultivated and to be free of extractables which could interfere with their growth.
3 Microbiological Methods for Monitoring the Environment, Water and Wastes, EPA/600/8-78/017. 1978. US EPA.
4 U.S. Geological Survey Techniques of Water-Resource Investigations, Book 5, Laboratory Analysis, Chapter A4, Methods for Collection and Analysis of Aquatic Biological and Microbiological Samples. 1989. USGS.
5 Because the MF technique usually yields low and variable recovery from chlorinated wastewaters, the Most Probable Number method will be required to resolve any controversies.
6 Tests must be conducted to provide organism enumeration (density). Select the appropriate configuration of tubes/filtrations and dilutions/volumes to account for the quality, character, consistency, and anticipated organism density of the water sample.
7 When the MF method has been used previously to test waters with high turbidity, large numbers of noncoliform bacteria, or samples that may contain organisms stressed by chlorine, a parallel test should be conducted with a multiple-tube technique to demonstrate applicability and comparability of results.
8 To assess the comparability of results obtained with individual methods, it is suggested that side-by-side tests be conducted across seasons of the year with the water samples routinely tested in accordance with the most current Standard Methods for the Examination of Water and Wastewater or EPA alternate test procedure (ATP) guidelines.
9 Annual Book of ASTM Standards—Water and Environmental Technology, Section 11.02. 2000, 1999, 1996. ASTM International.
10 Official Methods of Analysis of AOAC International. 16th Edition, 4th Revision, 1998. AOAC International.
11 Recommended for enumeration of target organism in sewage sludge.
12 The multiple-tube fermentation test is used in 9221B.2-2014. Lactose broth may be used in lieu of lauryl tryptose broth (LTB), if at least 25 parallel tests are conducted between this broth and LTB using the water samples normally tested, and this comparison demonstrates that the false-positive rate and false-negative rate for total coliform using lactose broth is less than 10 percent. No requirement exists to run the completed phase on 10 percent of all total coliform-positive tubes on a seasonal basis.
13 These tests are collectively known as defined enzyme substrate tests.
14 After prior enrichment in a presumptive medium for total coliform using 9221B.2-2014, all presumptive tubes or bottles showing any amount of gas, growth or acidity within 48 h ± 3 h of incubation shall be submitted to 9221F-2014. Commercially available EC-MUG media or EC media supplemented in the laboratory with 50 µg/mL of MUG may be used.
15 Method 1680: Fecal Coliforms in Sewage Sludge (Biosolids) by Multiple-Tube Fermentation Using Lauryl-Tryptose Broth (LTB) and EC Medium, EPA-821-R-14-009. September 2014. U.S. EPA.
16 Samples shall be enumerated by the multiple-tube or multiple-well procedure. Using multiple-tube procedures, employ an appropriate tube and dilution configuration of the sample as needed and report the Most Probable Number (MPN). Samples tested with Colilert® may be enumerated with the multiple-well procedures, Quanti-Tray® or Quanti-Tray®/2000 and the MPN calculated from the table provided by the manufacturer.
17 Colilert-18® is an optimized formulation of the Colilert® for the determination of total coliforms and E. coli that provides results within 18 h of incubation at 35 °C rather than the 24 h required for the Colilert® test and is recommended for marine water samples.
18 Descriptions of the Colilert®, Colilert-18®, Quanti-Tray®, and Quanti-Tray®/2000 may be obtained from IDEXX Laboratories, Inc.
19 A description of the mColiBlue24® test is available from Hach Company.
20 Method 1681: Fecal Coliforms in Sewage Sludge (Biosolids) by Multiple-Tube Fermentation Using A-1 Medium, EPA-821-R-06-013. July 2006. U.S. EPA.
21 Method 1603.1: Escherichia coli (E. coli) in Water by Membrane Filtration Using Modified membrane-Thermotolerant Escherichia coli Agar (Modified mTEC), EPA-821-R-23-008. September 2023. U.S. EPA.
22 Method 1682: Salmonella in Sewage Sludge (Biosolids) by Modified Semisolid Rappaport-Vassiliadis (MSRV) Medium, EPA-821-R-14-012. September 2014. U.S. EPA.
23 A description of the Enterolert® test may be obtained from IDEXX Laboratories Inc.
24 Method 1600.1: Enterococci in Water by Membrane Filtration Using Membrane-Enterococcus Indoxyl-β-D-Glucoside Agar (mEI), EPA-821-R-23-006. September 2023. U.S. EPA.
25 Methods for Measuring the Acute Toxicity of Effluents and Receiving Waters to Freshwater and Marine Organisms, EPA-821-R-02-012. Fifth Edition, October 2002. U.S. EPA; and U.S. EPA Whole Effluent Toxicity Methods Errata Sheet, EPA 821-R-02-012-ES. December 2016.
26 Short-term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, EPA-821-R-02-013. Fourth Edition, October 2002. U.S. EPA; and U.S. EPA Whole Effluent Toxicity Methods Errata Sheet, EPA 821-R-02-012-ES. December 2016.
27 Short-term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Marine and Estuarine Organisms, EPA-821-R-02-014. Third Edition, October 2002. U.S. EPA; and U.S. EPA Whole Effluent Toxicity Methods Errata Sheet, EPA 821-R-02-012-ES. December 2016.
28 To use Colilert-18® to assay for fecal coliforms, the incubation temperature is 44.5 ± 0.2 °C, and a water bath incubator is used.
29 On a monthly basis, at least ten blue colonies from positive samples must be verified using Lauryl Tryptose Broth and EC broth, followed by count adjustment based on these results; and representative non-blue colonies should be verified using Lauryl Tryptose Broth. Where possible, verifications should be done from randomized sample sources.
30 On a monthly basis, at least ten sheen colonies from positive samples must be verified using lauryl tryptose broth and brilliant green lactose bile broth, followed by count adjustment based on these results; and representative non-sheen colonies should be verified using lauryl tryptose broth. Where possible, verifications should be done from randomized sample sources.
31 Subject coliform positive samples determined by 9222 B-2015 or other membrane filter procedure to 9222 I-2015 using NA-MUG media.
32 Verification of colonies by incubation of BHI agar at 10 ± 0.5 °C for 48 ± 3 h is optional. As per the Errata to the 23rd Edition of Standard Methods for the Examination of Water and Wastewater “Growth on a BHI agar plate incubated at 10 ± 0.5 °C for 48 ± 3 h is further verification that the colony belongs to the genus Enterococcus.”
33 9221F. 2-2014 allows for simultaneous detection of E. coli and thermotolerant fecal coliforms by adding inverted vials to EC-MUG; the inverted vials collect gas produced by thermotolerant fecal coliforms.
Table IB—List of Approved Inorganic Test Procedures
| Parameter | Methodology 58 | EPA 52 | Standard methods 84 | ASTM | USGS/AOAC/Other | |
|---|---|---|---|---|---|---|
| 1. Acidity (as CaCO3), mg/L | Electrometric endpoint or phenolphthalein endpoint | 2310 B-2020 | D1067-16 | I-1020-85. 2 | ||
| 2. Alkalinity (as CaCO3), mg/L | Electrometric or Colorimetric titration to pH 4.5, Manual | 2320 B-2021 | D1067-16 | 973.43 3, I-1030-85. 2 | ||
| Automatic | 310.2 (Rev. 1974) 1 | I-2030-85. 2 | ||||
| 3. Aluminum—Total, 4 mg/L | Digestion, 4 followed by any of the following: | |||||
| AA direct aspiration 36 | 3111 D-2019 or 3111 E-2019 | I-3051-85. 2 | ||||
| AA furnace | 3113 B-2020. | |||||
| STGFAA | 200.9 Rev. 2.2 (1994). | |||||
| ICP/AES 36 | 200.5 Rev 4.2 (2003) 68, 200.7 Rev. 4.4 (1994) | 3120 B-2020 | D1976-20 | I-4471-97. 50 | ||
| ICP/MS | 200.8, Rev. 5.4 (1994) | 3125 B-2020 | D5673-16 | 993.14 3, I-4472-97. 81 | ||
| Direct Current Plasma (DCP) 36 | D4190-15 | See footnote. 34 | ||||
| Colorimetric (Eriochrome cyanine R) | 3500-Al B-2020. | |||||
| 4. Ammonia (as N), mg/L | Manual distillation 6 or gas diffusion (pH > 11), followed by any of the following: | 350.1 Rev. 2.0 (1993) | 4500-NH3 B-2021 | 973.49. 3 | ||
| Nesslerization | D1426-15 (A) | 973.49 3, I-3520-85. 2 | ||||
| Titration | 4500-NH3 C-2021. | |||||
| Electrode | 4500-NH3 D-2021 or E-2021 | D1426-15 (B) | ||||
| Manual phenate, salicylate, or other substituted phenols in Berthelot reaction-based methods | 4500-NH3 F-2021 | See footnote. 60 | ||||
| Automated phenate, salicylate, or other substituted phenols in Berthelot reaction-based methods | 350.1 30 Rev. 2.0 (1993) | 4500-NH3 G-2021, 4500-NH3 H-2021 | I-4523-85 2, I-2522-90. 80 | |||
| Automated electrode | See footnote. 7 | |||||
| Ion Chromatography | D6919-17 | |||||
| Automated gas diffusion, followed by conductivity cell analysis | Timberline Ammonia-001. 74 | |||||
| Automated gas diffusion followed by fluorescence detector analysis | FIAlab100. 82 | |||||
| 5. Antimony—Total, 4 mg/L | Digestion, 4 followed by any of the following: | |||||
| AA direct aspiration 36 | 3111 B-2019. | |||||
| AA furnace | 3113 B-2020. | |||||
| STGFAA | 200.9 Rev. 2.2 (1994). | |||||
| ICP/AES 36 | 200.5 Rev 4.2 (2003) 68, 200.7 Rev. 4.4 (1994) | 3120 B-2020 | D1976-20. | |||
| ICP/MS | 200.8, Rev. 5.4 (1994) | 3125 B-2020 | D5673-16 | 993.14 3, I-4472-97. 81 | ||
| 6. Arsenic—Total, 4 mg/L | Digestion, 4 followed by any of the following: | 206.5 (Issued 1978). 1 | ||||
| AA gaseous hydride | 3114 B-2020 or 3114 C-2020 | D2972-15 (B) | I-3062-85. 2 | |||
| AA furnace | 3113 B-2020 | D2972-15 (C) | I-4063-98. 49 | |||
| STGFAA | 200.9 Rev. 2.2 (1994). | |||||
| ICP/AES 36 | 200.5, Rev 4.2 (2003) 68, 200.7 Rev. 4.4 (1994) | 3120 B-2020 | D1976-20. | |||
| ICP/MS | 200.8, Rev. 5.4 (1994) | 3125 B-2020 | D5673-16 | 993.14 3, I-4020-05. 70 | ||
| Colorimetric (SDDC) | 3500-As B-2020 | D2972-15 (A) | I-3060-85. 2 | |||
| 7. Barium—Total, 4 mg/L | Digestion, 4 followed by any of the following: | |||||
| AA direct aspiration 36 | 3111 D-2019 | I-3084-85. 2 | ||||
| AA furnace | 3113 B-2020 | D4382-18. | ||||
| ICP/AES 36 | 200.5, Rev 4.2 (2003) 68, 200.7 Rev. 4.4 (1994) | 3120 B-2020 | I-4471-97. 50 | |||
| ICP/MS | 200.8 Rev. 5.4 (1994) | 3125 B-2020 | D5673-16 | 993.14 3, I-4472-97. 81 | ||
| DCP 36 | See footnote. 34 | |||||
| 8. Beryllium—Total, 4 mg/L | Digestion, 4 followed by any of the following: | |||||
| AA direct aspiration | 3111 D-2019 or 3111 E-2019 | D3645-15 (A) | I-3095-85. 2 | |||
| AA furnace | 3113 B-2020 | D3645-15 (B). | ||||
| STGFAA | 200.9, Rev. 2.2 (1994). | |||||
| ICP/AES | 200.5 Rev 4.2 (2003) 68, 200.7 Rev. 4.4 (1994) | 3120 B-2020 | D1976-20 | I-4471-97. 50 | ||
| ICP/MS | 200.8 Rev. 5.4 (1994) | 3125 B-2020 | D5673-16 | 993.14 3, I-4472-97. 81 | ||
| DCP | D4190-15 | See footnote. 34 | ||||
| Colorimetric (aluminon) | See footnote 61 | |||||
| 9. Biochemical oxygen demand (BOD5), mg/L | Dissolved Oxygen Depletion | 5210 B-2016 85 | 973.44 3 p. 17 9, I-1578-78 8, see footnote. 1063 | |||
| 10. Boron—Total, 37 mg/L | Colorimetric (curcumin) | 4500-B B-2011 | I-3112-85. 2 | |||
| ICP/AES | 200.5 Rev 4.2 (2003) 68, 200.7 Rev. 4.4 (1994) | 3120 B-2020 | D1976-20 | I-4471-97. 50 | ||
| ICP/MS | 200.8 Rev. 5.4 (1994) | 3125 B-2020 | D5673-16 | 993.14. 3 | ||
| DCP | D4190-15 | See footnote. 34 | ||||
| 11. Bromide, mg/L | Electrode | D1246-16 | I-1125-85. 2 | |||
| Ion Chromatography | 300.0 Rev 2.1 (1993), and 300.1 Rev 1.0 (1997) | 4110 B-2020, C-2020 or D-2020 | D4327-17 | 993.30 3, I-2057-85. 79 | ||
| CIE/UV | 4140 B-2020 | D6508-15 | D6508 Rev. 2. 54 | |||
| 12. Cadmium—Total, 4 mg/L | Digestion, 4 followed by any of the following: | |||||
| AA direct aspiration 36 | 3111 B-2019 or 3111 C-2019 | D3557-17 (A or B) | 974.27 3 p. 37 9, I-3135-85 2 or I-3136-85. 2 | |||
| AA furnace | 3113 B-2020 | D3557-17 (D) | I-4138-89. 51 | |||
| STGFAA | 200.9 Rev. 2.2 (1994) | |||||
| ICP/AES 36 | 200.5 Rev 4.2 (2003) 68, 200.7 Rev. 4.4 (1994) | 3120 B-2020 | D1976-20 | I-1472-85 2 or I-4471-97. 50 | ||
| ICP/MS | 200.8, Rev. 5.4 (1994) | 3125 B-2020 | D5673-16 | 993.14 3, I-4472-97. 81 | ||
| DCP 36 | D4190-15 | See footnote. 34 | ||||
| Voltammetry 11 | D3557-17 (C). | |||||
| Colorimetric (Dithizone) | 3500-Cd D-1990. | |||||
| 13. Calcium—Total, 4 mg/L | Digestion 4 followed by any of the following: | |||||
| AA direct aspiration | 3111 B-2019 or 3111 D-2019 | D511-14 (B) | I-3152-85. 2 | |||
| ICP/AES | 200.5 Rev 4.2 (2003) 68, 200.7 Rev. 4.4 (1994) | 3120 B-2020 | I-4471-97. 50 | |||
| ICP/MS | 200.8, Rev. 5.4 (1994) | 3125 B-2020 | D5673-16 | 993.14. 3 | ||
| DCP | See footnote. 34 | |||||
| Titrimetric (EDTA) | 3500-Ca B-2020 | D511-14 (A). | ||||
| Ion Chromatography | D6919-17. | |||||
| 14. Carbonaceous biochemical oxygen demand (CBOD5), mg/L 12 | Dissolved Oxygen Depletion with nitrification inhibitor | 5210 B-2016 85 | See footnotes. 35 63 | |||
| 15. Chemical oxygen demand (COD), mg/L | Titrimetric | 410.3 (Rev. 1978) 1 | 5220 B-2011 or C-2011 | D1252-06(12) (A) | 973.46 3 p. 17 9, I-3560-85. 2 | |
| Spectrophotometric, manual or automatic | 410.4 Rev. 2.0 (1993) | 5220 D-2011 | D1252-06(12) (B) | See footnotes 131483, I-3561-85. 2 | ||
| 16. Chloride, mg/L | Titrimetric: (silver nitrate) | 4500-Cl− B-2021 | D512-12 (B) | I-1183-85. 2 | ||
| (Mercuric nitrate) | 4500-Cl− C-2021 | D512-12 (A) | 973.51 3, I-1184-85. 2 | |||
| Colorimetric: manual | I-1187-85. 2 | |||||
| Automated (ferricyanide) | 4500-Cl− E-2021 | I-2187-85. 2 | ||||
| Potentiometric Titration | 4500-Cl− D-2021. | |||||
| Ion Selective Electrode | D512-12 (C). | |||||
| Ion Chromatography | 300.0 Rev 2.1 (1993), and 300.1 Rev 1.0 (1997) | 4110 B-2020 or 4110 C-2020 | D4327-17 | 993.30 3, I-2057-90. 51 | ||
| CIE/UV | 4140 B-2020 | D6508-15 | D6508, Rev. 2. 54 | |||
| 17. Chlorine—Total residual, mg/L | Amperometric direct | 4500-Cl D-2011 | D1253-14. | |||
| Amperometric direct (low level) | 4500-Cl E-2011. | |||||
| Iodometric direct | 4500-Cl B-2011. | |||||
| Back titration ether end-point 15 | 4500-Cl C-2011. | |||||
| DPD-FAS | 4500-Cl F-2011. | |||||
| Spectrophotometric, DPD | 4500-Cl G-2011. | |||||
| Electrode | See footnote. 16 | |||||
| 17A. Chlorine—Free Available, mg/L | Amperometric direct | 4500-Cl D-2011 | D1253-14 | |||
| Amperometric direct (low level) | 4500-Cl E-2011. | |||||
| DPD-FAS | 4500-Cl F-2011. | |||||
| Spectrophotometric, DPD | 4500-Cl G-2011. | |||||
| 18. Chromium VI dissolved, mg/L | 0.45-micron filtration followed by any of the following: | |||||
| AA chelation-extraction | 3111 C-2019 | I-1232-85. 2 | ||||
| Ion Chromatography | 218.6 Rev. 3.3 (1994) | 3500-Cr C-2020 | D5257-17 | 993.23. 3 | ||
| Colorimetric (diphenyl-carbazide) | 3500-Cr B-2020 | D1687-17 (A) | I-1230-85. 2 | |||
| 19. Chromium—Total, 4 mg/L | Digestion, 4 followed by any of the following: | |||||
| AA direct aspiration 36 | 3111 B-2019 | D1687-17 (B) | 974.27 3, I-3236-85. 2 | |||
| AA chelation-extraction | 3111 C-2019. | |||||
| AA furnace | 3113 B-2020 | D1687-17 (C) | I-3233-93. 46 | |||
| STGFAA | 200.9 Rev. 2.2 (1994). | |||||
| ICP/AES 36 | 200.5 Rev 4.2 (2003) 68, 200.7 Rev. 4.4 (1994) | 3120 B-2020 | D1976-20. | |||
| ICP/MS | 200.8 Rev. 5.4 (1994) | 3125 B-2020 | D5673-16 | 993.14 3, I-4020-05 70 I-4472-97. 81 | ||
| DCP 36 | D4190-15 | See footnote. 34 | ||||
| Colorimetric (diphenyl-carbazide) | 3500-Cr B-2020. | |||||
| 20. Cobalt—Total, 4 mg/L | Digestion, 4 followed by any of the following: | |||||
| AA direct aspiration | 3111 B-2019 or 3111 C-2019 | D3558-15 (A or B) | p. 37 9, I-323985. 2 | |||
| AA furnace | 3113 B-2020 | D3558-15 (C) | I-4243-89. 51 | |||
| STGFAA | 200.9 Rev. 2.2 (1994). | |||||
| ICP/AES | 200.7 Rev. 4.4 (1994) | 3120 B-2020 | D1976-20 | I-4471-97. 50 | ||
| ICP/MS | 200.8 Rev. 5.4 (1994) | 3125 B-2020 | D5673-16 | 993.14 3, I-4020-05 70 I-4472-97. 81 | ||
| DCP | D4190-15 | See footnote. 34 | ||||
| 21. Color, platinum cobalt units or dominant wavelength, hue, luminance purity | Colorimetric (ADMI) | 2120 F-2021. 78 | ||||
| Platinum cobalt visual comparison | 2120 B-2021 | I-1250-85. 2 | ||||
| Spectrophotometric | See footnote. 18 | |||||
| 22. Copper—Total, 4 mg/L | Digestion, 4 followed by any of the following: | |||||
| AA direct aspiration 36 | 3111 B-2019 or 3111 C-2019 | D1688-17 (A or B) | 974.27 3 p. 37 9, I-3270-85 2 or I-3271-85. 2 | |||
| AA furnace | 3113 B-2020 | D1688-17 (C) | I-4274-89. 51 | |||
| STGFAA | 200.9 Rev. 2.2 (1994). | |||||
| ICP/AES 36 | 200.5 Rev 4.2 (2003), 68 200.7 Rev. 4.4 (1994) | 3120 B-2020 | D1976-20 | I-4471-97. 50 | ||
| ICP/MS | 200.8 Rev. 5.4 (1994) | 3125 B-2020 | D5673-16 | 993.14 3, I-4020-05 70, I-4472-97. 81 | ||
| DCP 36 | D4190-15 | See footnote. 34 | ||||
| Colorimetric (Neocuproine) | 3500-Cu B-2020. | |||||
| Colorimetric (Bathocuproine) | 3500-Cu C-2020 | See footnote. 19 | ||||
| 23. Cyanide—Total, mg/L | Automated UV digestion/distillation and Colorimetry | Kelada-01. 55 | ||||
| Segmented Flow Injection, In-Line Ultraviolet Digestion, followed by gas diffusion amperometry | 4500-CN− P-2021 | D7511-12 (17). | ||||
| Manual distillation with MgCl2, followed by any of the following: | 335.4 Rev. 1.0 (1993) 57 | 4500-CN− B-2021 and C-2021 | D2036-09(15)(A), D7284-20 | 10-204-00-1-X. 56 | ||
| Flow Injection, gas diffusion amperometry | D2036-09(15)(A) D7284-20. | |||||
| Titrimetric | 4500-CN− D-2021 | D2036-09(15)(A) | See footnote 9 p. 22. | |||
| Spectrophotometric, manual | 4500-CN− E-2021 | D2036-09(15)(A) | I-3300-85. 2 | |||
| Semi-Automated 20 | 335.4 Rev. 1.0 (1993) 57 | 4500-CN− N-2021 | 10-204-00-1-X 56, I-4302-85. 2 | |||
| Ion Chromatography | D2036-09(15)(A). | |||||
| Ion Selective Electrode | 4500-CN− F-2021 | D2036-09(15)(A). | ||||
| 24. Cyanide—Available, mg/L | Cyanide Amenable to Chlorination (CATC); Manual distillation with MgCl2, followed by Titrimetric or Spectrophotometric | 4500-CN− G-2021 | D2036-09(15)(B). | |||
| Flow injection and ligand exchange, followed by gas diffusion amperometry 59 | 4500-CN− Q-2021 | D6888-16 | OIA-1677-09. 44 | |||
| Automated Distillation and Colorimetry (no UV digestion) | Kelada-01. 55 | |||||
| 24A. Cyanide—Free, mg/L | Flow Injection, followed by gas diffusion amperometry | 4500-CN− R-2021 | D7237-18 (A) | OIA-1677-09. 44 | ||
| Manual micro-diffusion and colorimetry | D4282-15. | |||||
| 25. Fluoride—Total, mg/L | Manual distillation, 6 followed by any of the following: | 4500-F− B-2021 | D1179-16 (A). | |||
| Electrode, manual | 4500-F− C-2021 | D1179-16 (B). | ||||
| Electrode, automated | 4500-F− G-2021 | I-4327-85. 2 | ||||
| Colorimetric, (SPADNS) | 4500-F− D-2021. | |||||
| Automated complexone | 4500-F− E-2021. | |||||
| Ion Chromatography | 300.0 Rev 2.1 (1993) and 300.1 Rev 1.0 (1997) | 4110 B-2020 or C-2020 | D4327-17 | 993.30. 3 | ||
| CIE/UV | 4140 B-2020 | D6508-15 | D6508, Rev. 2. 54 | |||
| 26. Gold—Total, 4 mg/L | Digestion, 4 followed by any of the following: | |||||
| AA direct aspiration | 3111 B-2019. | |||||
| AA furnace | 231.2 (Issued 1978) 1 | 3113 B-2020. | ||||
| ICP/MS | 200.8, Rev. 5.4 (1994) | 3125 B-2020 | D5673-16 | 993.14. 3 | ||
| DCP | See footnote. 34 | |||||
| 27. Hardness—Total (as CaCO(3), mg/L | Automated colorimetric | 130.1 (Issued 1971). 1 | ||||
| Titrimetric (EDTA) | 2340 C-2021 | D1126-17 | 973.52B 3, I-1338-85. 2 | |||
| Ca plus Mg as their carbonates, by any approved method for Ca and Mg (See Parameters 13 and 33), provided that the sum of the lowest point of quantitation for Ca and Mg is below the NPDES permit requirement for Hardness. | 2340 B-2021. | |||||
| 28. Hydrogen ion (pH), pH units | Electrometric measurement | 4500-H + B-2021 | D1293-18 (A or B) | 973.41 3, I-1586-85. 2 | ||
| Automated electrode | 150.2 (Dec. 1982) 1 | See footnote 21 I-2587-85. 2 | ||||
| 29. Iridium—Total, 4 mg/L | Digestion, 4 followed by any of the following: | |||||
| AA direct aspiration | 3111 B-2019. | |||||
| AA furnace | 235.2 (Issued 1978). 1 | |||||
| ICP/MS | 3125 B-2020. | |||||
| 30. Iron—Total, 4 mg/L | Digestion, 4 followed by any of the following: | |||||
| AA direct aspiration 36 | 3111 B-2019 or 3111 C-2019 | D1068-15 (A) | 974.27 3, I-3381-85. 2 | |||
| AA furnace | 3113 B-2020 | D1068-15 (B). | ||||
| STGFAA | 200.9, Rev. 2.2 (1994). | |||||
| ICP/AES 36 | 200.5 Rev. 4.2 (2003) 68, 200.7 Rev. 4.4 (1994) | 3120 B-2020 | D1976-20 | I-4471-97. 50 | ||
| ICP/MS | 200.8, Rev. 5.4 (1994) | 3125 B-2020 | D5673-16 | 993.14. 3 | ||
| DCP 36 | D4190-15 | See footnote. 34 | ||||
| Colorimetric (Phenanthroline) | 3500-Fe B-2011 | D1068-15 (C) | See footnote. 22 | |||
| 31. Kjeldahl Nitrogen 5—Total (as N), mg/L | Manual digestion 20 and distillation or gas diffusion, followed by any of the following: | 4500-Norg B-2021 or C-2021 and 4500-NH3 B-2021 | D3590-17 (A) | I-4515-91. 45 | ||
| Titration | 4500-NH3 C-2021 | 973.48. 3 | ||||
| Nesslerization | D1426-15 (A). | |||||
| Electrode | 4500-NH3 D-2021 or E-2021 | D1426-15 (B). | ||||
| Semi-automated phenate | 350.1 Rev. 2.0 (1993) | 4500-NH3 G-2021 or 4500-NH3 H-2021. | ||||
| Manual phenate, salicylate, or other substituted phenols in Berthelot reaction based methods | 4500-NH3 F-2021 | See footnote. 60 | ||||
| Automated gas diffusion, followed by conductivity cell analysis | Timberline Ammonia-001. 74 | |||||
| Automated gas diffusion followed by fluorescence detector analysis | FIAlab 100. 82 | |||||
| Automated Methods for TKN that do not require manual distillation | ||||||
| Automated phenate, salicylate, or other substituted phenols in Berthelot reaction-based methods colorimetric (auto digestion and distillation) | 351.1 (Rev. 1978) 1 | I-4551-78. 8 | ||||
| Semi-automated block digestor colorimetric (distillation not required) | 351.2 Rev. 2.0 (1993) | 4500-Norg D-2021 | D3590-17 (B) | I-4515-91. 45 | ||
| Block digester, followed by Auto distillation and Titration | See footnote. 39 | |||||
| Block digester, followed by Auto distillation and Nesslerization | See footnote. 40 | |||||
| Block Digester, followed by Flow injection gas diffusion (distillation not required) | See footnote. 41 | |||||
| Digestion with peroxdisulfate, followed by Spectrophotometric (2,6-dimethyl phenol) | Hach 10242. 76 | |||||
| Digestion with persulfate, followed by Colorimetric | NCASI TNTP W10900. 77 | |||||
| 32. Lead—Total, 4 mg/L | Digestion, 4 followed by any of the following: | |||||
| AA direct aspiration 36 | 3111 B-2019 or 3111 C-2019 | D3559-15 (A or B) | 974.27 3, I-3399-85. 2 | |||
| AA furnace | 3113 B-2020 | D3559-15 (D) | I-4403-89. 51 | |||
| STGFAA | 200.9 Rev. 2.2 (1994). | |||||
| ICP/AES 36 | 200.5 Rev. 4.2 (2003) 68, 200.7 Rev. 4.4 (1994) | 3120 B-2020 | D1976-20 | I-4471-97. 50 | ||
| ICP/MS | 200.8 Rev. 5.4 (1994) | 3125 B-2020 | D5673-16 | 993.14 3, I-4472-97. 81 | ||
| DCP 36 | D4190-15 | See footnote. 34 | ||||
| Voltammetry 11 | D3559-15 (C). | |||||
| Colorimetric (Dithizone) | 3500-Pb B-2020. | |||||
| 33. Magnesium—Total, 4 mg/L | Digestion, 4 followed by any of the following: | |||||
| AA direct aspiration | 3111 B-2019 | D511-14 (B) | 974.27 3, I-3447-85. 2 | |||
| ICP/AES | 200.5 Rev. 4.2 (2003) 68, 200.7 Rev. 4.4 (1994) | 3120 B-2020 | D1976-20 | I-4471-97. 50 | ||
| ICP/MS | 200.8 Rev. 5.4 (1994) | 3125 B-2020 | D5673-16 | 993.14. 3 | ||
| DCP | See footnote. 34 | |||||
| Ion Chromatography | D6919-17. | |||||
| 34. Manganese—Total, 4 mg/L | Digestion, 4 followed by any of the following: | |||||
| AA direct aspiration 36 | 3111 B-2019 or 3111 C-2019 | D858-17 (A or B) | 974.27 3, I-3454-85. 2 | |||
| AA furnace | 3113 B-2020 | D858-17 (C). | ||||
| STGFAA | 200.9 Rev. 2.2 (1994). | |||||
| ICP/AES 36 | 200.5, Rev. 4.2 (2003) 68; 200.7, Rev. 4.4 (1994) | 3120 B-2020 | D1976-20 | I-4471-97. 50 | ||
| ICP/MS | 200.8 Rev. 5.4 (1994) | 3125 B-2020 | D5673-16 | 993.14 3, I-4472-97. 81 | ||
| DCP 36 | D4190-15 | See footnote. 34 | ||||
| Colorimetric (Persulfate) | 3500-Mn B-2020 | 920.203. 3 | ||||
| Colorimetric (Periodate) | See footnote. 23 | |||||
| 35. Mercury—Total, mg/L | Cold vapor, Manual | 245.1 Rev. 3.0 (1994) | 3112 B-2020 | D3223-17 | 977.22 3, I-3462-85. 2 | |
| Cold vapor, Automated | 245.2 (Issued 1974). 1 | |||||
| Cold vapor atomic fluorescence spectrometry (CVAFS) | 245.7 Rev. 2.0 (2005) 17 | I-4464-01. 71 | ||||
| Purge and Trap CVAFS | 1631E. 43 | |||||
| 36. Molybdenum—Total, 4 mg/L | Digestion, 4 followed by any of the following: | |||||
| AA direct aspiration | 3111 D-2019 | I-3490-85. 2 | ||||
| AA furnace | 3113 B-2020 | I-3492-96. 47 | ||||
| ICP/AES | 200.7 Rev. 4.4 (1994) | 3120 B-2020 | D1976-20 | I-4471-97. 50 | ||
| ICP/MS | 200.8 Rev. 5.4 (1994) | 3125 B-2020 | D5673-16 | 993.14 3, I-4472-97. 81 | ||
| DCP | See footnote. 34 | |||||
| 37. Nickel—Total, 4 mg/L | Digestion, 4 followed by any of the following: | |||||
| AA direct aspiration 36 | 3111 B-2019 or 3111 C-2019 | D1886-14 (A or B) | I-3499-85. 2 | |||
| AA furnace | 3113 B-2020 | D1886-14 (C) | I-4503-89. 51 | |||
| STGFAA | 200.9 Rev. 2.2 (1994). | |||||
| ICP/AES 36 | 200.5 Rev. 4.2 (2003) 68, 200.7 Rev. 4.4 (1994) | 3120 B-2020 | D1976-20 | I-4471-97. 50 | ||
| ICP/MS | 200.8, Rev. 5.4 (1994) | 3125 B-2020 | D5673-16 | 993.14 3, I-4020-05 70, I-4472-97. 81 | ||
| DCP 36 | D4190-15 | See footnote. 34 | ||||
| 38. Nitrate (as N), mg/L | Ion Chromatography | 300.0 Rev. 2.1 (1993) and 300.1 Rev. 1.0 (1997) | 4110 B-2020 or C-2020 | D4327-17 | 993.30. 3 | |
| CIE/UV | 4140 B-2020 | D6508-15 | D6508, Rev. 2. 54 | |||
| Ion Selective Electrode | 4500-NO3− D-2019. | |||||
| Colorimetric (Brucine sulfate) | 352.1 (Issued 1971) 1 | 973.50 3, 419D 86, p. 28. 9 | ||||
| Spectrophotometric (2,6-dimethylphenol) | Hach 10206. 75 | |||||
| Nitrate-nitrite N minus Nitrite N (see parameters 39 and 40). | ||||||
| 39. Nitrate-nitrite (as N), mg/L | Cadmium reduction, Manual | 4500-NO3− E-2019 | D3867-16 (B). | |||
| Cadmium reduction, Automated | 353.2 Rev. 2.0 (1993) | 4500-NO3− F-2019 or 4500-NO3− I-2019 | D3867-16 (A) | I-2545-90. 51 | ||
| Automated hydrazine | 4500-NO3− H-2019. | |||||
| Reduction/Colorimetric | See footnote. 62 | |||||
| Ion Chromatography | 300.0 Rev. 2.1 (1993) and 300.1 Rev. 1.0 (1997) | 4110 B-2020 or C-2020 | D4327-17 | 993.30. 3 | ||
| CIE/UV | 4140 B-2020 | D6508-15 | D6508, Rev. 2. 54 | |||
| Enzymatic reduction, followed by automated colorimetric determination | D7781-14 | I-2547-11 72, I-2548-11 72, N07-0003. 73 | ||||
| Enzymatic reduction, followed by manual colorimetric determination | 4500-NO3− J-2018. | |||||
| Spectrophotometric (2,6-dimethylphenol) | Hach 10206. 75 | |||||
| 40. Nitrite (as N), mg/L | Spectrophotometric: Manual | 4500-NO2− B-2021 | See footnote. 25 | |||
| Automated (Diazotization) | I-4540-85 2 see footnote 62, I-2540-90. 80 | |||||
| Automated (*bypass cadmium reduction) | 353.2 Rev. 2.0 (1993) | 4500-NO3− F-2019, 4500-NO3− I-2019 | D3867-16 (A) | I-4545-85. 2 | ||
| Manual (*bypass cadmium or enzymatic reduction) | 4500-NO3− E-2019, 4500-NO3− J-2018 | D3867-16 (B). | ||||
| Ion Chromatography | 300.0 Rev. 2.1 (1993) and 300.1 Rev. 1.0 (1997) | 4110 B-2020 or C-2020 | D4327-17 | 993.30. 3 | ||
| CIE/UV | 4140 B-2020 | D6508-15 | D6508, Rev. 2. 54 | |||
| Automated (*bypass Enzymatic reduction) | D7781-14 | I-2547-11 72, I-2548-11 72, N07-0003. 73 | ||||
| 41. Oil and grease—Total recoverable, mg/L | Hexane extractable material (HEM): n-Hexane extraction and gravimetry | 1664 Rev. A 1664 Rev. B 42 | 5520 B or G-2021. 38 | |||
| Silica gel treated HEM (SGT-HEM): Silica gel treatment and gravimetry | 1664 Rev. A, 1664 Rev. B 42 | 5520 B or G-2021 38 and 5520 F-2021. 38 | ||||
| 42. Organic carbon—Total (TOC), mg/L | Combustion | 5310 B-2014 | D7573-18a e1 | 973.47 3, p. 14 24 | ||
| Heated persulfate or UV persulfate oxidation | 5310 C-2014, 5310 D-2011 | D4839-03(17) | 973.47 3, p. 14 24 | |||
| 43. Organic nitrogen (as N), mg/L | Total Kjeldahl N (Parameter 31) minus ammonia N (Parameter 4). | |||||
| 44. Ortho-phosphate (as P), mg/L | Ascorbic acid method: | |||||
| Automated | 365.1 Rev. 2.0 (1993) | 4500-P F-2021 or G-2021 | 973.56 3, I-4601-85 2, I-2601-90. 80 | |||
| Manual, single-reagent | 4500-P E-2021 | D515-88 (A) | 973.55. 3 | |||
| Manual, two-reagent | 365.3 (Issued 1978). 1 | |||||
| Ion Chromatography | 300.0 Rev. 2.1 (1993) and 300.1 Rev. 1.0 (1997) | 4110 B-2020 or C-2020 | D4327-17 | 993.30. 3 | ||
| CIE/UV | 4140 B-2020 | D6508-15 | D6508, Rev. 2. 54 | |||
| 45. Osmium—Total 4, mg/L | Digestion, 4 followed by any of the following: | |||||
| AA direct aspiration | 3111 D-2019. | |||||
| AA furnace | 252.2 (Issued 1978). 1 | |||||
| 46. Oxygen, dissolved, mg/L | Winkler (Azide modification) | 4500-O (B-F)-2021 | D888-18 (A) | 973.45B 3, I-1575-78. 8 | ||
| Electrode | 4500-O G-2021 | D888-18 (B) | I-1576-78. 8 | |||
| Luminescence-Based Sensor | 4500-O H-2021 | D888-18 (C) | See footnotes. 63, 64 | |||
| 47. Palladium—Total, 4 mg/L | Digestion, 4 followed by any of the following: | |||||
| AA direct aspiration | 3111 B-2019. | |||||
| AA furnace | 253.2 (Issued 1978). 1 | |||||
| ICP/MS | 3125 B-2020. | |||||
| DCP | See footnote. 34 | |||||
| 48. Phenols, mg/L | Manual distillation, 26 followed by any of the following: | 420.1 (Rev. 1978) 1 | 5530 B-2021 | D1783-01(12) | ||
| Colorimetric (4AAP) manual | 420.1 (Rev. 1978) 1 | 5530 D-2021 27 | D1783-01(12) (A or B). | |||
| Automated colorimetric (4AAP) | 420.4 Rev. 1.0 (1993). | |||||
| 49. Phosphorus (elemental), mg/L | Gas-liquid chromatography | See footnote. 28 | ||||
| 50. Phosphorus—Total, mg/L | Digestion, 20 followed by any of the following: | 4500-P B (5)-2021 | 973.55. 3 | |||
| Manual | 365.3 (Issued 1978) 1 | 4500-P E-2021 | D515-88 (A). | |||
| Automated ascorbic acid reduction | 365.1 Rev. 2.0 (1993) | 4500-P (F-H)-2021 | 973.56 3, I-4600-85. 2 | |||
| ICP/AES 436 | 200.7 Rev. 4.4 (1994) | 3120 B-2020 | I-4471-97. 50 | |||
| Semi-automated block digestor (TKP digestion) | 365.4 (Issued 1974) 1 | D515-88 (B) | I-4610-91. 48 | |||
| Digestion with persulfate, followed by Colorimetric | NCASI TNTP W10900. 77 | |||||
| 51. Platinum—Total, 4 mg/L | Digestion, 4 followed by any of the following: | |||||
| AA direct aspiration | 3111 B-2019. | |||||
| AA furnace | 255.2 (Issued 1978). 1 | |||||
| ICP/MS | 3125 B-2020. | |||||
| DCP | See footnote. 34 | |||||
| 52. Potassium—Total, 4 mg/L | Digestion, 4 followed by any of the following: | |||||
| AA direct aspiration | 3111 B-2019 | 973.5 3, I-3630-85. 2 | ||||
| ICP/AES | 200.7 Rev. 4.4 (1994) | 3120 B-2020. | ||||
| ICP/MS | 200.8, Rev. 5.4 (1994) | 3125 B-2020 | D5673-16 | 993.14. 3 | ||
| Flame photometric | 3500-K B-2020. | |||||
| Electrode | 3500-K C-2020. | |||||
| Ion Chromatography | D6919-17. | |||||
| 53. Residue—Total, mg/L | Gravimetric, 103-105° | 2540 B-2020 | I-3750-85. 2 | |||
| 54. Residue—filterable, mg/L | Gravimetric, 180° | 2540 C-2020 | D5907-18 (B) | I-1750-85. 2 | ||
| 55. Residue—non-filterable (TSS), mg/L | Gravimetric, 103-105° post-washing of residue | 2540 D-2020 | D5907-18 (A) | I-3765-85. 2 | ||
| 56. Residue—settleable, mg/L | Volumetric (Imhoff cone), or gravimetric | 2540 F-2020. | ||||
| 57. Residue—Volatile, mg/L | Gravimetric, 550° | 160.4 (Issued 1971) 1 | 2540 E-2020 | I-3753-85. 2 | ||
| 58. Rhodium—Total, 4 mg/L | Digestion, 4 followed by any of the following: | |||||
| AA direct aspiration, or | 3111 B-2019. | |||||
| AA furnace | 265.2 (Issued 1978). 1 | |||||
| ICP/MS | 3125 B-2020. | |||||
| 59. Ruthenium—Total, 4 mg/L | Digestion, 4 followed by any of the following: | |||||
| AA direct aspiration, or | 3111 B-2019. | |||||
| AA furnace | 267.2. 1 | |||||
| ICP/MS | 3125 B-2020. | |||||
| 60. Selenium—Total, 4 mg/L | Digestion, 4 followed by any of the following: | |||||
| AA furnace | 3113 B-2020 | D3859-15 (B) | I-4668-98. 49 | |||
| STGFAA | 200.9 Rev. 2.2 (1994). | |||||
| ICP/AES 36 | 200.5 Rev 4.2 (2003) 68, 200.7 Rev. 4.4 (1994) | 3120 B-2020 | D1976-20. | |||
| ICP/MS | 200.8 Rev. 5.4 (1994) | 3125 B-2020 | D5673-16 | 993.14 3, I-4020-05 70 I-4472-97. 81 | ||
| AA gaseous hydride | 3114 B-2020, or 3114 C-2020 | D3859-15 (A) | I-3667-85. 2 | |||
| 61. Silica—Dissolved, 37 mg/L | 0.45-micron filtration followed by any of the following: | |||||
| Colorimetric, Manual | 4500-SiO2 C-2021 | D859-16 | I-1700-85. 2 | |||
| Automated (Molybdosilicate) | 4500-SiO2 E-2021 or F-2021 | I-2700-85. 2 | ||||
| ICP/AES | 200.5 Rev. 4.2 (2003), 68 200.7 Rev. 4.4 (1994) | 3120 B-2020 | I-4471-97. 50 | |||
| ICP/MS | 200.8 Rev. 5.4 (1994) | 3125 B-2020 | D5673-16 | 993.14. 3 | ||
| 62. Silver—Total, 4 31 mg/L | Digestion, 4 29 followed by any of the following: | |||||
| AA direct aspiration | 3111 B-2019 or 3111 C-2019 | 974.27 3, p. 37 9, I-3720-85. 2 | ||||
| AA furnace | 3113 B-2020 | I-4724-89. 51 | ||||
| STGFAA | 200.9 Rev. 2.2 (1994). | |||||
| ICP/AES | 200.5 Rev. 4.2 (2003) 68, 200.7 Rev. 4.4 (1994) | 3120 B-2020 | D1976-20 | I-4471-97. 50 | ||
| ICP/MS | 200.8 Rev. 5.4 (1994) | 3125 B-2020 | D5673-16 | 993.14 3, I-4472-97. 81 | ||
| DCP | See footnote. 34 | |||||
| 63. Sodium—Total, 4 mg/L | Digestion, 4 followed by any of the following: | |||||
| AA direct aspiration | 3111 B-2019 | 973.54 3, I-3735-85. 2 | ||||
| ICP/AES | 200.5 Rev. 4.2 (2003) 68, 200.7 Rev. 4.4 (1994) | 3120 B-2020 | I-4471-97. 50 | |||
| ICP/MS | 200.8 Rev. 5.4 (1994) | 3125 B-2020 | D5673-16 | 993.14. 3 | ||
| DCP | See footnote. 34 | |||||
| Flame photometric | 3500-Na B-2020. | |||||
| Ion Chromatography | D6919-17. | |||||
| 64. Specific conductance, micromhos/cm at 25 °C | Wheatstone bridge | 120.1 (Rev. 1982) 1 | 2510 B-2021 | D1125-95(99) (A) | 973.40 3, I-2781-85. 2 | |
| 65. Sulfate (as SO4), mg/L | Automated colorimetric | 375.2 Rev. 2.0 (1993) | 4500-SO42− F-2021 or G-2021. | |||
| Gravimetric | 4500-SO42− C-2021 or D-2021 | 925.54. 3 | ||||
| Turbidimetric | 4500-SO42− E-2021 | D516-16. | ||||
| Ion Chromatography | 300.0 Rev. 2.1 (1993) and 300.1 Rev. 1.0 (1997) | 4110 B-2020 or C-2020 | D4327-17 | 993.30 3, I-4020-05. 70 | ||
| CIE/UV | 4140 B-2020 | D6508-15 | D6508 Rev. 2. 54 | |||
| 66. Sulfide (as S), mg/L | Sample Pretreatment | 4500-S 2− B, C-2021. | ||||
| Titrimetric (iodine) | 4500-S 2− F-2021 | I-3840-85. 2 | ||||
| Colorimetric (methylene blue) | 4500-S 2− D-2021. | |||||
| Ion Selective Electrode | 4500-S 2− G-2021 | D4658-15. | ||||
| 67. Sulfite (as SO3), mg/L | Titrimetric (iodine-iodate) | 4500-SO32− B-2021. | ||||
| 68. Surfactants, mg/L | Colorimetric (methylene blue) | 5540 C-2021 | D2330-20. | |||
| 69. Temperature, °C | Thermometric | 2550 B-2010 | See footnote. 32 | |||
| 70. Thallium-Total, 4 mg/L | Digestion, 4 followed by any of the following: | |||||
| AA direct aspiration | 3111 B-2019. | |||||
| AA furnace | 279.2 (Issued 1978) 1 | 3113 B-2020. | ||||
| STGFAA | 200.9 Rev. 2.2 (1994). | |||||
| ICP/AES | 200.7 Rev. 4.4 (1994) | 3120 B-2020 | D1976-20. | |||
| ICP/MS | 200.8, Rev. 5.4 (1994) | 3125 B-2020 | D5673-16 | 993.14 3, I-4471-97 50 I-4472-97. 81 | ||
| 71. Tin—Total, 4 mg/L | Digestion, 4 followed by any of the following: | |||||
| AA direct aspiration | 3111 B-2019 | I-3850-78. 8 | ||||
| AA furnace | 3113 B-2020. | |||||
| STGFAA | 200.9 Rev. 2.2 (1994). | |||||
| ICP/AES | 200.5 Rev. 4.2 (2003) 68, 200.7 Rev. 4.4 (1994). | |||||
| ICP/MS | 200.8 Rev. 5.4 (1994) | 3125 B-2020 | D5673-16 | 993.14. 3 | ||
| 72. Titanium—Total, 4 mg/L | Digestion, 4 followed by any of the following: | |||||
| AA direct aspiration | 3111 D-2019. | |||||
| AA furnace | 283.2 (Issued 1978). 1 | |||||
| ICP/AES | 200.7 Rev. 4.4 (1994). | |||||
| ICP/MS | 200.8 Rev. 5.4 (1994) | 3125 B-2020 | D5673-16 | 993.14. 3 | ||
| DCP | See footnote. 34 | |||||
| 73. Turbidity, NTU 53 | Nephelometric | 180.1, Rev. 2.0 (1993) | 2130 B-2020 | D1889-00 | I-3860-85 2, see footnotes. 656667 | |
| 74. Vanadium—Total, 4 mg/L | Digestion, 4 followed by any of the following: | |||||
| AA direct aspiration | 3111 D-2019. | |||||
| AA furnace | 3113 B-2020 | D3373-17. | ||||
| ICP/AES | 200.5 Rev. 4.2 (2003) 68, 200.7 Rev. 4.4 (1994) | 3120 B-2020 | D1976-20 | I-4471-97. 50 | ||
| ICP/MS | 200.8 Rev. 5.4 (1994) | 3125 B-2020 | D5673-16 | 993.14 3, I-4020-05. 70 | ||
| DCP | D4190-15 | See footnote. 34 | ||||
| Colorimetric (Gallic Acid) | 3500-V B-2011. | |||||
| 75. Zinc—Total, 4 mg/L | Digestion, 4 followed by any of the following: | |||||
| AA direct aspiration 36 | 3111 B-2019 or 3111 C-2019 | D1691-17 (A or B) | 974.27 3 p. 37 9, I-3900-85. 2 | |||
| AA furnace | 289.2 (Issued 1978). 1 | |||||
| ICP/AES 36 | 200.5 Rev. 4.2 (2003), 68 200.7, Rev. 4.4 (1994) | 3120 B-2020 | D1976-20 | I-4471-97. 50 | ||
| ICP/MS | 200.8 Rev. 5.4 (1994) | 3125 B-2020 | D5673-16 | 993.14 3, I-4020-05 70, I-4472-97. 81 | ||
| DCP 36 | D4190-15 | See footnote. 34 | ||||
| Colorimetric (Zincon) | 3500 Zn B-2020 | See footnote. 33 | ||||
| 76. Acid Mine Drainage | 1627. 69 | |||||